Re: Thoughts on Axioimager line of Microscopes/ Imaging system

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Thoughts-on-Axioimager-line-of-Microscopes-Imaging-system-tp592349p592363.html

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Hi Farid,

The deconvolution is painfully slow, especially the "best" mode. This
is with the PC they sold us, 4 Gb ram, Windows XP. Even after 20
hours it may not be done. There is no way to save in progress, so
either the job needs to be cancelled or no one can use the scope
until the job is done (and no time estimate to completion).

I like the 3D rendering module. Had a rendered dithizone fluorescent
pancreatic islet for Nature Protocol's web site image of the week a
couple of weeks ago.

There are some strange bugs in AxioVision's (4/2007 release) macros
control of my Axiovert 200M's filter cubes and shutters.

George


At 01:08 PM 2/27/2008, you wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>Hello All,
>I would appreciate hearing thoughts on Zeiss' line of Axioimager
>imaging systems. I've just found out that an A1 (most basic, for
>documentation) is going into our tiny (2 scope) facility. However, I
>am thinking of asking for an upgrade to the M1, motorized and
>apparently suitable for live cell imaging, 3D deconvolution
>microscopy, FRET even. Is anyone out there using their Axiovision's
>for live cell imaging, FRET, 3D acquisition and deconvolution?
>
>Any and all thoughts are greatly appreciated.
>Cheers
>Farid
>
>--
>Farid Jalali MSc
>Senior Research Technician/ Lab Manager
>Dr. Robert Bristow Lab
>Applied Molecular Oncology
>Princess Margaret Hospital
>Toronto, Canada
>416-946-4501 X4351 (Princess Margaret Hospital)
>416-581-7754 STTARR at MaRS Building
>416-581-7791 STTARR Microscopy Suite






George McNamara, Ph.D.
University of Miami, Miller School of Medicine
Image Core
Miami, FL 33010
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http://home.earthlink.net/~geomcnamara/
http://www.sylvester.org/research/SR_lab_analytical.asp?ana=desc 
(Analytical Imaging Core Facility)