Hi Farid, we just received an Observer Z1
with the intention of setting up a live-cell imaging station. We do not yet
have our water immersion objectives or our C9100-13 EMCCD, so I cannot comment
on functionality as of yet. However, I would certainly get the M1 and would get
the Z1 if you can. I know these get expensive real quick, but I think you will thank
yourself for the scanning stage later. My idea is to use the docking station
away from the scope free from vibration and interruption as we acquire in an
automated motorized fashion within an enclosed microscope incubator. Reaching
in the incubator hundreds of times does not sound very appealing for live-cell
imaging. Moreover, I think you will use the motorized functions for
autofocusing and the like during long-term acquisitions. Furthermore, it is the
motorized components that really supply the functionality to the docking
station.
Cheers,
Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of
626-359-8111 x62872
From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of Farid Jalali
Sent: Wednesday, February 27, 2008
10:08 AM
To: [hidden email]
Subject: Thoughts on Axioimager
line of Microscopes/ Imaging system
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hello All,
I would appreciate hearing thoughts on Zeiss' line of Axioimager
imaging systems. I've just found out that an A1 (most basic, for
documentation) is going into our tiny (2 scope) facility. However, I am
thinking of asking for an upgrade to the M1, motorized and apparently suitable
for live cell imaging, 3D deconvolution microscopy, FRET even. Is anyone out
there using their Axiovision's for live cell imaging, FRET, 3D acquisition and
deconvolution?
Any and all thoughts are greatly appreciated.
Cheers
Farid
--
Farid Jalali MSc
Senior Research Technician/ Lab Manager
Dr. Robert Bristow Lab
Applied Molecular Oncology
416-946-4501 X4351 (
416-581-7754 STTARR at
416-581-7791 STTARR Microscopy Suite
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