2P standard

Posted by Boswell, Carl A - (cboswell) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/2P-standard-tp5923717.html

Hi all,
I'm looking for some trustworthy, permanent standard(s) to use in a MP system to monitor the condition of detectors and the system in general.   The goal is a fluorescent source that is immutable.  This could done either by having unrestricted replacement of bleached label with unbleached label (i.e. a solution), or a uniform and very large solid, so that it is unlikely that any one tiny volume will be imaged repeatedly.   We've tried several iterations of an aqueous solution of label, but sealing the prep does not prevent eventual evaporation.  Plus, there seems to be some modification of FITC, for example,  in solution over time (a month) regardless of exposure to light.  I'm not sure I can trust the consistency of colored plastic slides, since they are not manufactured with this role in mind.  One possibility is uranyl glass slides, but Corning says they don't make these any more.  Does anyone know a source?  We're also considering quantum dots in something like immersion oil , in a sealed chamber but I don't have any experience with these labels in a hydrophobic environment.  

Thanks for your insights.
C


Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
Univ. of Arizona
520-954-7053
FAX [phone]