Re: 2P standard

Posted by Mark Cannell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/2P-standard-tp5923717p5923826.html

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How about Fluorescent plastic slides -I got some free from Chroma.

Cheers


On 15/01/2011, at 12:34 PM, Boswell, Carl A - (cboswell) wrote:

> Hi all,
> I'm looking for some trustworthy, permanent standard(s) to use in a  
> MP system to monitor the condition of detectors and the system in  
> general.   The goal is a fluorescent source that is immutable.  This  
> could done either by having unrestricted replacement of bleached  
> label with unbleached label (i.e. a solution), or a uniform and very  
> large solid, so that it is unlikely that any one tiny volume will be  
> imaged repeatedly.   We've tried several iterations of an aqueous  
> solution of label, but sealing the prep does not prevent eventual  
> evaporation.  Plus, there seems to be some modification of FITC, for  
> example,  in solution over time (a month) regardless of exposure to  
> light.  I'm not sure I can trust the consistency of colored plastic  
> slides, since they are not manufactured with this role in mind.  One  
> possibility is uranyl glass slides, but Corning says they don't make  
> these any more.  Does anyone know a source?  We're also considering  
> quantum dots in something like immersion oil , in a sealed chamber  
> but I don't have any experience with these labels in a hydrophobic  
> environment.
>
> Thanks for your insights.
> C
>
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> Univ. of Arizona
> 520-954-7053
> FAX [phone]
>
>