Re: mCherry

Posted by Michael Weber-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Alexa-Fluor-488-solubility-problem-tp592502p592517.html

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Dear Alby,

check the Svoboda paper: PLOS Biology in 2006 vol. 4 (11) pp. e370 (Rapid
redistribution of synaptic PSD-95 in the neocortex in vivo.). They used a
fixed wavelength 1030 nm laser for mCherry (110 mW in bfp). We tried ~1050
nm on Spectraphysics MaiTai laser, but power was too low (while still
getting nice images with 559 nm single photon). An Ultra II should give a
bit more power in this range, however, still not much (200 mW at laser
head). So either the mentioned laser from Amplitude Systems, or maybe this
OPO unit.

Michael

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Ciao
> Is there anyone working with 2PE and mCherry? In case, do you have
> excitation spectrum, a real one?
> All  the best
> Alby
>
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