Re: Fluorophore bleaching by excitation light sources

Posted by Stanislav Vitha on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorophore-bleaching-by-excitation-light-sources-tp592521p592523.html

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Regarding the difference between LED illumination and Hg lamp -
Perhaps if the illumination is not continuous but is set up for strobe
operation of the LED, this could allow dark state relaxation an prolong
the life of the fluorophore (I am not sure the same would apply to the
life of the cell). - The time between light pulses should be more than one
microscecond.
reference:  
Gerald Donnert, Christian Eggeling & Stefan W Hell: Major signal increase
in fluorescence microscopy through dark-state relaxation. Nature Methods -
4, 81 - 86 (2007)


Stan Vitha