Re: Fluorophore bleaching by excitation light sources

Posted by Mark Cannell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorophore-bleaching-by-excitation-light-sources-tp592521p592531.html

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I don't understand this. The only explanation I can think of is that
that the excitation wavelengths are _not_ the same in the two cases.(if
they were and the power is the same the photon flux is the same).  Any
other comments/views?

Regards.

Gerard Whoriskey wrote:

> Commercial interest.
>
> We have recently had preliminary feedback from a number of independent
> sources that show much reduced bleaching when a sample is excited using an
> LED source rather than a Hg bulb. These tests, carried out with identical
> powers in the excitation bandpass region, showed that imaging could be
> carried out for up to three times longer.
> On live tests cells were seen to be still living happily after being
> exposed for twice the time it took to kill the cells under Hg excitation.
> We are still gathering information on this and intend to publish in due
> course.
>