Posted by
Ignatius, Mike on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorophore-bleaching-by-excitation-light-sources-tp592521p592535.html
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalMy understanding is that it is critical that all UV light be
removed/blocked as Hg bulbs produce lots of UV. Diodes don't have this
concern.
Not all scopes can claim 100% UV block produced by Hg or xenon bulbs.
But when it is done (for example AP/Delta Vision Scopes), cell viability
along with dye stability is greatly enhanced.
Mike Ignatius
-----Original Message-----
From: Confocal Microscopy List [mailto:
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Behalf Of Mark Cannell
Sent: Tuesday, March 11, 2008 2:02 PM
To:
[hidden email]
Subject: Re: Fluorophore bleaching by excitation light sources
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalI don't understand this. The only explanation I can think of is that
that the excitation wavelengths are _not_ the same in the two cases.(if
they were and the power is the same the photon flux is the same). Any
other comments/views?
Regards.
Gerard Whoriskey wrote:
> Commercial interest.
>
> We have recently had preliminary feedback from a number of independent
> sources that show much reduced bleaching when a sample is excited
using an
> LED source rather than a Hg bulb. These tests, carried out with
identical
> powers in the excitation bandpass region, showed that imaging could be
> carried out for up to three times longer.
> On live tests cells were seen to be still living happily after being
> exposed for twice the time it took to kill the cells under Hg
excitation.
> We are still gathering information on this and intend to publish in
due
> course.
>