Confocal Microscopy List

Re: Far-red filter set

Posted by Schwartz, Owen (NIH/NIAID) [E] on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Far-red-filter-set-tp592652p592654.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Far-red filter set

I have observed as well that people in general cannot see out into the far red wavelengths. Sometimes if the sample is very bright, you can see it, but in most cases not. The far-red cubes do work very well however for most ccd cameras. Be aware that many cameras have a blue filter installed to reduce IR reaching the ccd. This might have to be removed (consult manufacturer) in order to image in the far-red.

Cheers,

Owen

Owen M. Schwartz PhD
Head, Biological Imaging Facility
NIAID-NIH
Bldg 4 room B2-30
4 Center Drive
Bethesda, MD 20892
Ph 301-402-5883
Fax 301-480-2882

From: Kenneth Bowitz Larsen [mailto:[hidden email]]
Sent: Friday, March 07, 2008 6:43 AM
To: [hidden email]
Subject: Far-red filter set

Hi,

we're considering buying a Y5 filter cube from Leica to see the far-red Alexas, DRAQ5 etc. but then someone mentioned to me that many (most?) people are unable to see far-red light. Does anyone know if this is true, or have experience with this (or a similar) filter cube? It has a BP 700/75 suppression filter.

Kenneth