Fixing GFP positive tissue after freeze sectioning

Posted by Hege Avsnes Dale on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Far-red-filter-set-tp592652p592656.html

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Hi.
Do anyone have a protocol for good preservation of GFP in tissue after
freeze sectioning?
The tissue is a tumor with EGFP-expressing cells and need only a
fixation that will preserve GFP (no permabilization  needed). Would it
also be a better idea to fix before sectioning?

Hege