Re: Far-red filter set

Posted by Mayandi Sivaguru on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Far-red-filter-set-tp592652p592664.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Kenneth, in my experience, using a M200, D640/20 (Ex), D680/30 (Em), 660 DCLP (BS), a spot size of around 10 micron diameter having an 8 bit intensity of over 200 is clearly visible to my eyes as well as to our graduate student. But   when you project this image to MRm camera (1388x1040) we typically set a exposure of 30-50 ms (which yield spot intensity of over 200 in 8 bits).
Shiv
 

At 05:42 AM 3/7/2008, you wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi,

we're considering buying a Y5 filter cube from Leica to see the far-red Alexas, DRAQ5 etc. but then someone mentioned to me that many (most?) people are unable to see far-red light. Does anyone know if this is true, or have experience with this (or a similar) filter cube? It has a BP 700/75 suppression filter.

Kenneth

Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

Office: 217.333.1214
Fax: [phone]
[hidden email]
http://core.igb.uiuc.edu