Confocal Microscopy List

Re: Far-red filter set

Posted by Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Far-red-filter-set-tp592652p592674.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Far-red filter set

We were recently imaging bacteria labelled with Atto 647. Nobody was

able to see them by eye, though the signal was excellent in confocal,

particularly with APD detection. This was a Leica filter set - I suppose

it was Y5 but don't know for sure. But the light source may not have been

ideal for excitation either.

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Peter Humphreys
Sent: Friday, 7 March 2008 11:03 PM
To: [hidden email]
Subject: Re: Far-red filter set

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We have a y5, can see 594, cy5 etc.

Dark red, strong signal more apparent when viewing, haven’t met anyone yet who can’t see it.

Peter

Imaging Facility Manager

Wellcome Trust Centre for Stem Cell Research

University of Cambridge

Tennis Court Road

Cambridge

CB2 1QR

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kenneth Bowitz Larsen
Sent: 07 March 2008 11:43
To: [hidden email]
Subject: Far-red filter set

Hi,

we're considering buying a Y5 filter cube from Leica to see the far-red Alexas, DRAQ5 etc. but then someone mentioned to me that many (most?) people are unable to see far-red light. Does anyone know if this is true, or have experience with this (or a similar) filter cube? It has a BP 700/75 suppression filter.

Kenneth

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