pH and oxygen gradients

Posted by Patrizia Camelliti on
URL: http://confocal-microscopy-list.275.s1.nabble.com/pH-and-oxygen-gradients-tp592712.html

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Dear all,

I'd like to measure pH gradients and/or oxygen gradients in living tissue
slices about 350 micron thick. Does anybody know a good method? Is there
any dye I could use with a 2-photon (wavelenght 700-990)? Will the dye be
able to reach the deep layers of cells in the slice?

Thanks,
Patrizia

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Dr Patrizia Camelliti
Department of Physiology, Anatomy and Genetics
University of Oxford, UK