Re: Not a confocal question

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Hi, Vaishali-
One of my students found that all of our tumor lines (from rat
respiratory tract) rounded up on glass.  The normal lines didn't seem
to care whether they were on glass or plastic.  At least they looked
the same - we use a coated coverslip now.  I vacuum deposit a metal
(platinum is good, germanium, or silicon itself seem to work about as
well).  Then, the cells treat the surface just like tissue culture
plastic.
C.

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear All
>We are working with B16F10 melanoma cell line  which
>is a highly adhesive line , recently we are facing the
>problem of growing them . When we grow them in petri
>plates it spreads very well but when seeded on
>coverslips it remains rounded. What could be the
>possible reason.
>Regards
>Vaishali
>
>
>
>____________________________________________________________________________________
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--
Carol A. Heckman, Ph.D.
Director, Center for Microscopy & Microanalysis
and Professor of Biological Sciences
Bowling Green State University
Bowling Green, OH 43403
USA
website: http://www.bgsu.edu/departments/biology/facilities/MnM