Nipkow Disk Pinholes question

Posted by O'Malley, Donald on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nipkow-Disk-Pinholes-question-tp592789.html

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 Hi Folks,

I am wondering if the pinhole-aperture diameter in the Nipkow
disk can be changed in instances where one might wish to
open the aperture to trade away some z-resolution in
exchange for increased signal?  I imagine this could be done
by switching between disks with different sized pinholes, but
am not sure if this would be fast and easy enough such that
one could go "back and forth" during e.g. a physiological
experiment.  This seems an area where traditional point-
scanning confocals may have an advantage.

Also, are there different disks for different magnification
objectives?  Is the right disk automatically selected on
such instruments?

thanks for any info.

Don O'Malley
Northeastern University