Posted by
Rietdorf, Jens on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nipkow-Disk-Pinholes-question-tp592789p592790.html
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi Don,
Another way to change the pinhole size than changing the disc is to
change the magnification of your microscope, because pinholes are
projected by the microscope optics. We use an optovar on our Yokogawa
SD. Bear in mind changing mag will also affect sampling frequency in
camera based systems.
Cheers, jens
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On
Behalf Of Donald M. O'Malley
Sent: Donnerstag, 15. Mai 2008 22:07
To:
[hidden email]
Subject: Nipkow Disk Pinholes question
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Folks,
I am wondering if the pinhole-aperture diameter in the Nipkow disk can
be changed in instances where one might wish to open the aperture to
trade away some z-resolution in exchange for increased signal? I
imagine this could be done by switching between disks with different
sized pinholes, but am not sure if this would be fast and easy enough
such that one could go "back and forth" during e.g. a physiological
experiment. This seems an area where traditional point- scanning
confocals may have an advantage.
Also, are there different disks for different magnification objectives?
Is the right disk automatically selected on such instruments?
thanks for any info.
Don O'Malley
Northeastern University