Re: Nipkow Disk Pinholes question

Posted by RICHARD BURRY on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nipkow-Disk-Pinholes-question-tp592789p592792.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Don
 
This is a problem with the Nipkow (Yokawaga) spinning disk, it is optomized for N.A. 1.40 which makes is difficult to use for lower mag objectives.  We are currently setting up the Visitech Infinity3, a 2D Array scanning confocal that has 7 different sets of pinholes.  This technology appears to have an advantage over the spinning disk and maintain the highest possible resolution. 
 
You also might consider the Olympus DU spinning disk with the changable disks for different objectives.  This technology is different from the Nipkow disk using glass disks with squares for transmitted light.  The DU works well with low mag objectives and bright samples.
 
Dick

Richard W. Burry, Ph.D.
Department of Neuroscience, College of Medicine
Campus Microscopy and Imaging Facility, Director
The Ohio State University

----- Original Message -----
From: "Donald M. O'Malley" <[hidden email]>
Date: Thursday, May 15, 2008 4:07 pm
Subject: Nipkow Disk Pinholes question
To: [hidden email]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>  Hi Folks,
>
> I am wondering if the pinhole-aperture diameter in the Nipkow
> disk can be changed in instances where one might wish to
> open the aperture to trade away some z-resolution in
> exchange for increased signal?  I imagine this could be done
> by switching between disks with different sized pinholes, but
> am not sure if this would be fast and easy enough such that
> one could go "back and forth" during e.g. a physiological
> experiment.  This seems an area where traditional point-
> scanning confocals may have an advantage.
>
> Also, are there different disks for different magnification
> objectives?  Is the right disk automatically selected on
> such instruments?
>
> thanks for any info.
>
> Don O'Malley
> Northeastern University
>
>
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