Leica TCS SP2 Overlay images

Posted by Matthew Pearson-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Leica-TCS-SP2-Overlay-images-tp592854.html

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Hi Everyone,

Does anyone know why in the Leica Confocal Software when you scan multiple fluorophores you select the 'Ovl' button to get an overlay image and then you would press the Single scan button to take an image.  Yet in the Experiment window where the files are saved the overlay image is not saved only the 3 images of each fluorophore (in a triple label).  I have to actually right click the overlay image  and then 'send to experiment, selection raw' in order for it to be saved.  does anyone know if there is a setting to allow the overlay image to be saved without having to save each one manually?  It just seems like a slightly tedious process.

Many thanks,

Matt Pearson.