Hi,
I am a Leica SP2 AOBS user and I feel Leica has got it correct. The data is collected as 8 or 12 bit greyscale in 1-4 channels. This is how the data should be saved, there are many programs that can analyze or merge this data. Overlay works well on the Lieca and if you really want to save it you can. Just remember that trying to extract channel information from an overlay image can be very difficult depending on how many channels there are and the color of the LUT’s.
Michael Weis
Electron Microscopy & Digital Imaging
Pacific Agri-Food Research Centre
Agriculture and Agri-Food Canada
4200 Highway 97
Summerland, BC V0H 1Z0
Telephone: 250-494-6410
Facsimile: 250-494-0755
P Before printing think about the environment. / Avant d'imprimer, il faut penser à l'environnement.
-----Original Message-----
From: Confocal Microscopy List
[mailto:[hidden email]] On
Behalf Of Matthew Pearson
Sent: Thursday, April 10, 2008
2:38 AM
To: [hidden email]
Subject: Leica TCS SP2 Overlay
images
Hi Everyone,
Does anyone know why in the Leica Confocal Software when you scan multiple
fluorophores you select the 'Ovl' button to get an overlay image and then you
would press the Single scan button to take an image. Yet in the
Experiment window where the files are saved the overlay image is not saved only
the 3 images of each fluorophore (in a triple label). I have to actually
right click the overlay image and then 'send to experiment, selection
raw' in order for it to be saved. does anyone know if there is a setting
to allow the overlay image to be saved without having to save each one manually?
It just seems like a slightly tedious process.
Many thanks,
Matt Pearson.
-- Imaging TechnicianCell Biology DivisionInstitute of OphthalmologyUniversity College LondonEC1V 9EL020 7608 6857[hidden email] | Free forum by Nabble | Edit this page |