Posted by
Michael Weber-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Leica-TCS-SP2-Overlay-images-tp592854p592856.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalMatt,
for which type of processing do you need the raw overlay? Overlays are
usually done by the software and not saved (at least not in raw format).
If I remember correctly, you can export the overlay from the experiments
list as ordinary tif or jpg. I am not sure about LCS, but it works in
LAS AF.
Michael
Matthew Pearson wrote:
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> Hi Everyone,
>
> Does anyone know why in the Leica Confocal Software when you scan
> multiple fluorophores you select the 'Ovl' button to get an overlay
> image and then you would press the Single scan button to take an image.
> Yet in the Experiment window where the files are saved the overlay image
> is not saved only the 3 images of each fluorophore (in a triple label).
> I have to actually right click the overlay image and then 'send to
> experiment, selection raw' in order for it to be saved. does anyone
> know if there is a setting to allow the overlay image to be saved
> without having to save each one manually? It just seems like a slightly
> tedious process.
>
> Many thanks,
>
> Matt Pearson.
>
>
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