Re: Staining of antibodies...

Posted by Ignatius, Mike on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Staining-of-antibodies-tp592882p592883.html

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We designed those microscale labeling kits for 20 to 100ugs.  50 should be fine.

 

Mike Ignatius

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Benoit Labarthe
Sent: Tuesday, May 06, 2008 11:55 AM
To: [hidden email]
Subject: Staining of antibodies...

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear All,

I have to stain antibodies with an antibody staining kit designed for 100µg of protein from molecular probes, my problem is that I can not spend 100µg of my precious antibody for this staining... Do you think that staining of only 50 µg of antibody with this kit would be OK? Do you have any tips to improve the staining of low concentration/mass of antibodies with this type of staining kit?

Thank you

benoit



--
Benoît Labarthe, PharmD, MSc
Etudiant PhD - PhD trainee
Institut de Cardiologie de Montréal - Montreal Heart Institute
Centre de Recherche, Research Center
Laboratoire du Dr.Théroux
5000 Bélanger Est
Montréal Québec Canada H1T 1C8
Tel [phone] ext 3017
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