Re: Staining of antibodies...
Posted by
Benoit Labarthe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Staining-of-antibodies-tp592882p592884.html
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Good news! what are the critical points of the protocol, would an increase of the incubation time to 2h help for the staining?
benoit
On Tue, May 6, 2008 at 3:12 PM, Ignatius, Mike <
[hidden email]> wrote:
We designed those microscale labeling kits for 20 to
100ugs. 50 should be fine.
Mike Ignatius
From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Benoit
Labarthe
Sent: Tuesday, May 06, 2008 11:55 AMSubject: Staining of
antibodies...
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear All,
I have
to stain antibodies with an antibody staining kit designed for 100µg of protein
from molecular probes, my problem is that I can not spend 100µg of my precious
antibody for this staining... Do you think that staining of only 50 µg of
antibody with this kit would be OK? Do you have any tips to improve the staining
of low concentration/mass of antibodies with this type of staining
kit?
Thank you
benoit
--
Benoît
Labarthe, PharmD, MSc
Etudiant PhD - PhD trainee
Institut de Cardiologie
de Montréal - Montreal Heart Institute
Centre de Recherche, Research
Center
Laboratoire du Dr.Théroux
5000 Bélanger Est
Montréal Québec
Canada H1T 1C8
Tel (514)-376 3330 ext 3017
Fax (514)- 376 1076
--
Benoît Labarthe, PharmD, MSc
Etudiant PhD - PhD trainee
Institut de Cardiologie de Montréal - Montreal Heart Institute
Centre de Recherche, Research Center
Laboratoire du Dr.Théroux
5000 Bélanger Est
Montréal Québec Canada H1T 1C8
Tel (514)-376 3330 ext 3017
Fax (514)- 376 1076