Re: Staining of antibodies...

Posted by Benoit Labarthe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Staining-of-antibodies-tp592882p592886.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Thank you Scott

Sadly, we do not have this choice, our experiment does not allow us to use secondary stainings....

benoit

On Tue, May 6, 2008 at 3:02 PM, Scott Howell <[hidden email]> wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Benoit,

If I am understanding you correctly it sounds like you might be better
off using a secondary antibody to "tag" your antibody instead of
directly tagging your primary. In this manner you will use a lot less.
Hope this helps.

Scott J. Howell, Ph.D.
Manager, Imaging Module
Visual Sciences Research Center
Case Western Reserve University
2085 Adelbert Rd.
Institute of Pathology Room 106
Cleveland, Ohio 44106
216-368-2300
http://www.case.edu/med/vsrc/

----- Original Message -----
From: Benoit Labarthe <[hidden email]>
Date: Tuesday, May 6, 2008 2:55 pm
Subject: Staining of antibodies...
To: [hidden email]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear All,
>
> I have to stain antibodies with an antibody staining kit designed
> for 100µg
> of protein from molecular probes, my problem is that I can not
> spend 100µg
> of my precious antibody for this staining... Do you think that
> staining of
> only 50 µg of antibody with this kit would be OK? Do you have any
> tips to
> improve the staining of low concentration/mass of antibodies with
> this type
> of staining kit?
>
> Thank you
>
> benoit
>
>
>
> --
> Benoît Labarthe, PharmD, MSc
> Etudiant PhD - PhD trainee
> Institut de Cardiologie de Montréal - Montreal Heart Institute
> Centre de Recherche, Research Center
> Laboratoire du Dr.Théroux
> 5000 Bélanger Est
> Montréal Québec Canada H1T 1C8
> Tel [phone] ext 3017
> Fax (514)- 376 1076
>

--
Benoît Labarthe, PharmD, MSc
Etudiant PhD - PhD trainee
Institut de Cardiologie de Montréal - Montreal Heart Institute
Centre de Recherche, Research Center
Laboratoire du Dr.Théroux
5000 Bélanger Est
Montréal Québec Canada H1T 1C8
Tel [phone] ext 3017
Fax (514)- 376 1076