http://confocal-microscopy-list.275.s1.nabble.com/2P-standard-tp5923717p5929332.html
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> Hi Carl,
>
> Instead of using a bleachable fluorescent target, use something that
> makes great second harmonic generation (SHG). If you MP laser tunes
> from 690-1040 nm an SHG specimen will be usable from 395-520 nm
> (maybe you can convince NIH's NCRR to give all of us S10 money to
> add an OPO or OPA to our MP systems to extend the wavelength range).
>
> Besides sugar crystals, starch granules, collagen fibers (see Rich
> Cole's post), Scott Fraser's lab published a PNAS paper in 2010
> using nanoparticles for multicolor SHG-immunofluorescence (antibody
> conjugation procedure developed by a different Caltech lab and cited
> by Fraser and colleagues). They used specific types of particles
> from
http://www.nanoamor.com/products> Multicolor SHG worked because some types of nanoparticles
> perferentially underwent SHG in specific excitation wavelength
> range, whereas others had broad excitation range.
>
> Enjoy,
>
> George
>
>
>
> On 1/14/2011 6:34 PM, Boswell, Carl A - (cboswell) wrote:
>> Hi all,
>> I'm looking for some trustworthy, permanent standard(s) to use in a
>> MP system to monitor the condition of detectors and the system in
>> general. The goal is a fluorescent source that is immutable.
>> This could done either by having unrestricted replacement of
>> bleached label with unbleached label (i.e. a solution), or a
>> uniform and very large solid, so that it is unlikely that any one
>> tiny volume will be imaged repeatedly. We've tried several
>> iterations of an aqueous solution of label, but sealing the prep
>> does not prevent eventual evaporation. Plus, there seems to be
>> some modification of FITC, for example, in solution over time (a
>> month) regardless of exposure to light. I'm not sure I can trust
>> the consistency of colored plastic slides, since they are not
>> manufactured with this role in mind. One possibility is uranyl
>> glass slides, but Corning says they don't make these any more.
>> Does anyone know a source? We're also considering quantum dots in
>> something like immersion oil , in a sealed chamber but I don't have
>> any experience with these labels in a hydrophobic environment.
>>
>> Thanks for your insights.
>> C
>>
>>
>> Carl A. Boswell, Ph.D.
>> Molecular and Cellular Biology
>> Univ. of Arizona
>> 520-954-7053
>> FAX 520-621-3709
>>
>>
>>