http://confocal-microscopy-list.275.s1.nabble.com/Strange-artifact-in-z-series-tp592967p592968.html
Apologies for repeating the message. I hit the wrong button
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> Hi all,
>
> while performing z sections of mitochondria-stained living cells, I´m
> finding that the bottom part of the cell displays a brighter mitochondria
> fluoresecence, showing more contrast respect to cytosol than mitochondria in
> the top part of the cell. My system scans z from bottom to top. If it were
> simple bleaching due to laser light passing through the cell, I should
> observe that the bottom mitochondria are also less stained in a second
> series, but that´s not the case (the difference between top and bottom still
> there)
> Any idea to explain it?
>
> Thanks
>
> Pedro
>
>