Confocal Microscopy List

Re: Pollen grain

Posted by Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pollen-grain-tp593121p593128.html

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I think it's phenolics that are responsible for the fluorescence. It

is pretty broad but you can see different peaks (depending on the

pollen).

I had thought that spiky pollen grains would be a good TIRF test

sample but not so - the fluorescence is deep enough below the

surface to be out of TIRF range.

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
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     http://www.guycox.net

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shalin Mehta
Sent: Tuesday, 17 June 2008 10:08 AM
To: [hidden email]
Subject: Re: Pollen grain

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I also keep one of the pollen grain slides in the confocal room. When a user
says that there's something wrong with the scope, I ask them if they've checked
will the pollen slide. Since they inevitably haven't, I tell them to do that
first, and then come back if there's a problem with the microscope. Amazingly,
they rarely return.

This is interesting, do pollen's have nice excitation-emission properties? Do they have specific peaks or just broad excitation and emission? What would be the underlying biological organelle/molecule responsible for autofluorescence?

Cheers

Shalin

Kristi DeCourcy

--
~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
mobile: +65-90694182
blog: shalin.wordpress.com
~~~~~~~~~~~~~~~~~~~~~~~~~~
Bioimaging Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574
website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html

Liver Cancer Functional Genomics Lab, #6-05
National Cancer Centre, Singapore 169610
http://www.nccs.com.sg/researcher/02_04d.htm

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