Re: Pollen gr=?iso-8859-1?Q?=E0in_?= RI ?

Posted by Jeremy Adler on
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Does anyone have a plausible RI for pollen grains, or the RI of a medium that produces the best images ?
 
Jeremy Adler
Cell Biology
The Wenner-Gren Inst.
Arrhenius Laboratories E5
Stockholm University
Stockholm 106 91
Sweden

________________________________

From: Confocal Microscopy List on behalf of Guy Cox
Sent: Tue 6/17/2008 03:44
To: [hidden email]
Subject: Re: Pollen grain


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I think it's phenolics that are responsible for the fluorescence.  It
is pretty broad but you can see different peaks (depending on the
pollen).  
 
I had thought that spiky pollen grains would be a good TIRF test
sample but not so - the fluorescence is deep enough below the
surface to be out of TIRF range.
 
                                                                            Guy
 


Optical Imaging Techniques in Cell Biology
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Associate Professor Guy Cox, MA, DPhil(Oxon)
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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shalin Mehta
Sent: Tuesday, 17 June 2008 10:08 AM
To: [hidden email]
Subject: Re: Pollen grain


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        I also keep one of the pollen grain slides in the confocal room. When a user
        says that there's something wrong with the scope, I ask them if they've checked
        will the pollen slide. Since they inevitably haven't, I tell them to do that
        first, and then come back if there's a problem with the microscope. Amazingly,
        they rarely return.
       

 
 
This is  interesting, do pollen's have nice excitation-emission properties? Do they have specific peaks or just broad excitation and emission? What would be the underlying biological organelle/molecule responsible for autofluorescence?
 
Cheers
Shalin

       
        Kristi DeCourcy
       





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        Div of Bioengineering, NUS Singapore 117574
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