http://confocal-microscopy-list.275.s1.nabble.com/Pollen-grain-tp593121p593130.html
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> Does anyone have a plausible RI for pollen grains, or the RI of a medium that produces the best images ?
>
> Jeremy Adler
> Cell Biology
> The Wenner-Gren Inst.
> Arrhenius Laboratories E5
> Stockholm University
> Stockholm 106 91
> Sweden
>
> ________________________________
>
> From: Confocal Microscopy List on behalf of Guy Cox
> Sent: Tue 6/17/2008 03:44
> To:
[hidden email]
> Subject: Re: Pollen grain
>
>
> Search the CONFOCAL archive at
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> I think it's phenolics that are responsible for the fluorescence. It
> is pretty broad but you can see different peaks (depending on the
> pollen).
>
> I had thought that spiky pollen grains would be a good TIRF test
> sample but not so - the fluorescence is deep enough below the
> surface to be out of TIRF range.
>
> Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
>
http://www.guycox.com/optical.htm> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
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> ________________________________
>
> From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Shalin Mehta
> Sent: Tuesday, 17 June 2008 10:08 AM
> To:
[hidden email]
> Subject: Re: Pollen grain
>
>
> Search the CONFOCAL archive at
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>
>
> I also keep one of the pollen grain slides in the confocal room. When a user
> says that there's something wrong with the scope, I ask them if they've checked
> will the pollen slide. Since they inevitably haven't, I tell them to do that
> first, and then come back if there's a problem with the microscope. Amazingly,
> they rarely return.
>
>
>
>
> This is interesting, do pollen's have nice excitation-emission properties? Do they have specific peaks or just broad excitation and emission? What would be the underlying biological organelle/molecule responsible for autofluorescence?
>
> Cheers
> Shalin
>
>
> Kristi DeCourcy
>
>
>
>
>
>
> --
> ~~~~~~~~~~~~~~~~~~~~~~~~~
> Shalin Mehta
> mobile: +65-90694182
> blog: shalin.wordpress.com <
http://shalin.wordpress.com/>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~
> Bioimaging Lab, Block-E3A, #7-10
> Div of Bioengineering, NUS Singapore 117574
> website:
http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html>
> Liver Cancer Functional Genomics Lab, #6-05
> National Cancer Centre, Singapore 169610
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