Re: Pollen grain

Posted by Lewis B Coons (lcoons) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pollen-grain-tp593121p593131.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Randy:
Mr. Blaudow and Mr. Norman were married in San Francisco this evening.  Judy Cole was the best man, ring bearer, and maid of honor (she has video of the occasion).  I know you wish the couple well.  We will have a post wedding party (with wedding cake) at my house when they get back.
Lewis


On 6/16/08 8:44 PM, "Guy Cox" <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I think it's phenolics that are responsible for the fluorescence.  It
is pretty broad but you can see different peaks (depending on the
pollen).  

I had thought that spiky pollen grains would be a good TIRF test
sample but not so - the fluorescence is deep enough below the
surface to be out of TIRF range.

                                                                           Guy



Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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From: Confocal Microscopy List [[hidden email] On Behalf Of Shalin Mehta
Sent: Tuesday, 17 June 2008 10:08 AM
To: [hidden email]
Subject: Re: Pollen grain

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I  also keep one of the pollen grain slides in the confocal room. When a  user
says that there's something wrong with the scope, I ask them if  they've checked
will the pollen slide. Since they inevitably haven't, I  tell them to do that
first, and then come back if there's a problem with  the microscope. Amazingly,
they rarely return.

 
This is  interesting, do pollen's have nice excitation-emission properties? Do they have specific peaks or just broad excitation and emission? What would be the underlying biological organelle/molecule responsible for autofluorescence?
 
Cheers
Shalin

Kristi DeCourcy

 
 




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~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
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blog:  shalin.wordpress.com <http://shalin.wordpress.com>
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Bioimaging  Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574  
website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html

Liver  Cancer Functional Genomics Lab, #6-05
National Cancer Centre, Singapore  169610
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