Re: Pollen grain

Posted by Farid Jalali on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pollen-grain-tp593121p593132.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Group,
I am not sure if this is a simple mistake or a joke, but perhaps Dr. Coons should be made aware that his personal emails are being directed to microscopy folks around the world. If this is meant to be a gag, my apologies.
Farid

On Mon, Jun 16, 2008 at 10:12 PM, Lewis B Coons (lcoons) <[hidden email]> wrote:

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Hi Randy:
Mr. Blaudow and Mr. Norman were married in San Francisco this evening.  Judy Cole was the best man, ring bearer, and maid of honor (she has video of the occasion).  I know you wish the couple well.  We will have a post wedding party (with wedding cake) at my house when they get back.

Lewis


On 6/16/08 8:44 PM, "Guy Cox" <[hidden email]> wrote:

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I think it's phenolics that are responsible for the fluorescence.  It
is pretty broad but you can see different peaks (depending on the
pollen).  

I had thought that spiky pollen grains would be a good TIRF test
sample but not so - the fluorescence is deep enough below the
surface to be out of TIRF range.

                                                                           Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone [phone]     Fax [phone]
Mobile [phone]
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     http://www.guycox.net <http://www.guycox.net/>  

 

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From: Confocal Microscopy List [[hidden email] On Behalf Of Shalin Mehta
Sent: Tuesday, 17 June 2008 10:08 AM
To: [hidden email]
Subject: Re: Pollen grain

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I  also keep one of the pollen grain slides in the confocal room. When a  user
says that there's something wrong with the scope, I ask them if  they've checked
will the pollen slide. Since they inevitably haven't, I  tell them to do that
first, and then come back if there's a problem with  the microscope. Amazingly,
they rarely return.

 
This is  interesting, do pollen's have nice excitation-emission properties? Do they have specific peaks or just broad excitation and emission? What would be the underlying biological organelle/molecule responsible for autofluorescence?
 
Cheers
Shalin

Kristi DeCourcy

 
 




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Shalin Mehta
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blog:  shalin.wordpress.com <http://shalin.wordpress.com>

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--
Farid Jalali MSc
Senior Research Technician- Lab Manager
Applied Molecular Oncology/ Princess Margaret Hospital
STTARR Innovation Facility/ Radiation Medicine Program
Toronto, Canada
416-946-4501 X4351 (Princess Margaret Hospital)
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