Re: Pollen grain

Posted by Lewis B Coons (lcoons) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pollen-grain-tp593121p593133.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Bob certainly has a way with words.  The rabbits (2) will cost around $350; the ticks around $150.  I can stand for the ticks.  Trust me, we need to use 2 rabbits.
Mr. Blaudow and Mr. Norman were married in San Francisco this evening.  Judy Cole was the best man, ring bearer, and maid of honor (she has video of the occasion).  I know you wish the couple well.
Dr. Vance broke down during the visit from Dr. Bayer.  He will see her again soon about her future and about teaching the class in the Fall soon.
Dr. Meyer and the Dean have given the OK to Dr. Bayer (pending the gracious blessing of the provost) to search for the new Director of the IMC this Fall.  The position will be 75% in the Biology department and the rest in the IMC.  We will look for a Cell or Molecular Biologist.  Can I recommend you to serve on the search committee?
It is very hot here.  We are looking forward to the river rising (but not too much) from the midwestern floods.
Hope you are enjoying your summer,
Lewis



On 6/16/08 8:44 PM, "Guy Cox" <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I think it's phenolics that are responsible for the fluorescence.  It
is pretty broad but you can see different peaks (depending on the
pollen).  

I had thought that spiky pollen grains would be a good TIRF test
sample but not so - the fluorescence is deep enough below the
surface to be out of TIRF range.

                                                                           Guy



Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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From: Confocal Microscopy List [[hidden email] On Behalf Of Shalin Mehta
Sent: Tuesday, 17 June 2008 10:08 AM
To: [hidden email]
Subject: Re: Pollen grain

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I  also keep one of the pollen grain slides in the confocal room. When a  user
says that there's something wrong with the scope, I ask them if  they've checked
will the pollen slide. Since they inevitably haven't, I  tell them to do that
first, and then come back if there's a problem with  the microscope. Amazingly,
they rarely return.

 
This is  interesting, do pollen's have nice excitation-emission properties? Do they have specific peaks or just broad excitation and emission? What would be the underlying biological organelle/molecule responsible for autofluorescence?
 
Cheers
Shalin

Kristi DeCourcy

 
 




--  
~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
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blog:  shalin.wordpress.com <http://shalin.wordpress.com>
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Bioimaging  Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574  
website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html

Liver  Cancer Functional Genomics Lab, #6-05
National Cancer Centre, Singapore  169610
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