Posted by
Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/2P-standard-tp5923717p5931757.html
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On 16.01.2011 15:38, George McNamara wrote:
> Hi Carl,
>
> Instead of using a bleachable fluorescent target, use something that
> makes great second harmonic generation (SHG). If you MP laser tunes from
> 690-1040 nm an SHG specimen will be usable from 395-520 nm (maybe you
> can convince NIH's NCRR to give all of us S10 money to add an OPO or OPA
> to our MP systems to extend the wavelength range).
If you succeed in getting that OPO, you also can use it as a source for
THG (Third harmonic generation). A water-glass interface (water on glass
slide) will give you a bright and uniform signal if the system is well
aligned. It still will work only with your blue detectors, though (e.g.
1270 nm / 3 = 423 nm)
Steffen
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
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