Re: How to align polarization filters in two filter cubes

Posted by ChrisWilms on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Strange-artifact-in-confocal-Z-stack-tp593216p593223.html

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Barbara correctly pointed to the role of objectives. But the situation  
is worse: even perfectly strainless lenses will depolarize light. The  
process of focusing light causes this depolarization and it becomes a  
significant factor when using high NA objectives. For some literature  
on this you might want to have a look at:

Methods Cell Biology 1989;30:333-52.
"Fluorescence polarization microscopy."
Axelrod, D.

Biophysical Journal, Vol 26, 557-573,
"Carbocyanine dye orientation in red cell membrane studied by  
microscopic fluorescence polarization"
Axelrod, D.

Regards, Christian


--
Christian Wilms
Wolfson Institute for Biomedical Research
University College London
Gower Street
London, WC1E 6BT
United Kingdom