Averaging

Posted by Sarah Kefayati on
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Dear list,

 

I need to average over different images of the single beads which are acquired in the same depth but different locations.I don't have any software in order to do that by the image-processing techniques.

 

This is my approach:

I crop a single bead image.then I lower the size(to the half of the size) of the pixel by the zoom factor.next I plot the intensity vs. position graph and I assume that the peak of the graph represents the center of the bead.then I move the cropping box till I locate the highest intensity pixel to the pixel in the center of the box.next I zoom back again to the original size and save the image and by the option available in the software (fluview v. 5) I add the images together.I also use sixfold kalman averaging for each image. 

 

I appreciate any comment on this approach in order to do that more accurately or any other suggestion.

 

Regards

Sarah