Re: Alexa 546 vs 555

Posted by Danielle Crippen on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Alexa-546-vs-555-tp593244p593245.html

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We use alexa 555 standard with the 543 laser and it’s great—fairly bright and stable (for fixed sample labeling at least).  I would hesitate to use 546 with the 543 laser…having a look at the Invitrogen fluorescence spectra viewer (no commercial interest) it will show that 543 laser will excite alexa 546 at about 40% efficiency, but alexa 555 at about 70% efficiency….of course these measurements are done in particular buffers etc. etc…just something to consider…

 

Cheers,

 

d

 

Danielle Crippen

Morphology and Imaging Core Manager

Buck Institute for Age Research

8001 Redwood Blvd

Novato, CA 94945

415-209-2046

 

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL
Sent: Monday, July 28, 2008 1:53 PM
To: [hidden email]
Subject: Alexa 546 vs 555

 

Dear List

 

Which Alexa dye do people prefer for use with a 543 laser, Alexa 546 or 555? Thanks!

 

Mike Model

Kent State University