Re: Nuclear Stains

Posted by Vickery Trinkaus-Randall on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nuclear-Stains-tp593262p593263.html

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

you can use ethidium bromide with your laser

Sarah Aubut wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello Listers,
>
>I have seen a lot of posts discussing variouos nuclear stains, and I have a
>question for you all.
>I work with a Nikon Confocal system that has 3 lasers, a 405, 488 and 543. I
>am having trouble finding a good nuclear stain that I can use on living
>cells that will work with these lasers. I have been using hoescht, but it is
>not optimal excited with the 405 laser. Are there any alternatives that
>anyone knows of that can be used on living cells???
>
>Thanks for your help!
>Sarah Aubut
>
>
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