Re: Nuclear Stains

Posted by Marc Thibault on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nuclear-Stains-tp593262p593264.html

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Hi,
If not already mentionned, here is an article comparing various nuclear
dyes.
I've not had super results with Draq5 on live cells. Lots of non-specific
staining.
M

http://www3.interscience.wiley.com/journal/112783064/abstract


-----Message d'origine-----
De : Vickery Trinkaus-Randall [mailto:[hidden email]]
Envoyé : 11 juin 2008 09:54
À : [hidden email]
Objet : Re: Nuclear Stains

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you can use ethidium bromide with your laser

Sarah Aubut wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello Listers,
>
>I have seen a lot of posts discussing variouos nuclear stains, and I
>have a question for you all.
>I work with a Nikon Confocal system that has 3 lasers, a 405, 488 and
>543. I am having trouble finding a good nuclear stain that I can use on
>living cells that will work with these lasers. I have been using
>hoescht, but it is not optimal excited with the 405 laser. Are there
>any alternatives that anyone knows of that can be used on living cells???
>
>Thanks for your help!
>Sarah Aubut
>
>
>  
>