Re: Nuclear Stains

Posted by Elaine Kunze on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nuclear-Stains-tp593262p593265.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal The latest issue of Cytometry has several wonderful reviews of DNA dyes (Syto and DRAQ5) and their properties.....and amazingly it is available ON-LINE at
http://www3.interscience.wiley.com/journal/33945/home




At 08:57 AM 6/11/2008, you wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello Listers,

I have seen a lot of posts discussing variouos nuclear stains, and I have a
question for you all.
I work with a Nikon Confocal system that has 3 lasers, a 405, 488 and 543. I
am having trouble finding a good nuclear stain that I can use on living
cells that will work with these lasers. I have been using hoescht, but it is
not optimal excited with the 405 laser. Are there any alternatives that
anyone knows of that can be used on living cells???

Thanks for your help!
Sarah Aubut

Elaine Kunze
Cytometry Facility
Huck Institute of the Life Sciences
319 Life Sciences Building
Penn State University
University Park, PA 16802
http://www.huck.psu.edu/facilities/cytometry-up/
814-863-2762