Re: Nuclear Stains -- INDUSTRY RESPONSE

Posted by Kilgore, Jason on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nuclear-Stains-tp593262p593266.html

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**Industry Response**

 

Another option to the dye options that have been recommended is Hoechst 34580, which is readily excited at 405nm and can be used for both live and fixed-cell nuclear labeling the same as the UV-excited Hoechst dyes.

 

The excitation is between ~360-430nm.  Emission is between ~460-610nm.

 

This is sold by Molecular Probes / Invitrogen, product H21486:

 

Link: http://products.invitrogen.com/ivgn/en/US/adirect/invitrogen;PRODUCTSJSESSIONID=LzSDLRFPRmphx0Dd2wX3jpV83gNJ1LmT1chsFV2N7vw2vLP1K21f!696270182!1773655413?cmd=catProductDetail&entryPoint=adirect&productID=H21486&CID=Search-h21486&messageType=catProductDetail&showAddButton=true

 

The best live-cell green-fluorescent we sell is SYTO 14, product S7576:

 

http://products.invitrogen.com/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&messageType=catProductDetail&showAddButton=true&productID=S7576&CID=Search-s7576

 

Cheers,

 

Jason

 

Jason A. Kilgore

Invitrogen Corporation

Molecular Probes Labeling and Detection Technologies

Technical Service Scientist

Eugene, OR  97402-9132

phone: [phone]

fax:      [phone]

[hidden email]

Please visit our website:  www.probes.invitrogen.com

 

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elaine Kunze
Sent: Wednesday, June 11, 2008 6:06 AM
To: [hidden email]
Subject: Re: Nuclear Stains

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal The latest issue of Cytometry has several wonderful reviews of DNA dyes (Syto and DRAQ5) and their properties.....and amazingly it is available ON-LINE at
http://www3.interscience.wiley.com/journal/33945/home




At 08:57 AM 6/11/2008, you wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello Listers,

I have seen a lot of posts discussing variouos nuclear stains, and I have a
question for you all.
I work with a Nikon Confocal system that has 3 lasers, a 405, 488 and 543. I
am having trouble finding a good nuclear stain that I can use on living
cells that will work with these lasers. I have been using hoescht, but it is
not optimal excited with the 405 laser. Are there any alternatives that
anyone knows of that can be used on living cells???

Thanks for your help!
Sarah Aubut

Elaine Kunze
Cytometry Facility
Huck Institute of the Life Sciences
319 Life Sciences Building
Penn State University
University Park, PA 16802
http://www.huck.psu.edu/facilities/cytometry-up/
814-863-2762