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URL: http://confocal-microscopy-list.275.s1.nabble.com/Nuclear-Stains-tp593262p593268.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalSarah,
On that instrument, you likely could utilize Draq5. For multi-color imaging, you could optimally utilize your 543 laser for Draq5 excitation and hopefully in your case a long-pass emission filter is available. With those lasers you likely don't have a band-pass filter that is perfect for Draq5, but different versions of long-pass filters associated with your 543 line would work fine for this scenario. If you have any questions about this, feel free to contact me off-list and I'd be happy to help further.
Cheers,
--
Samuel A. Connell
Director of Light Microscopy
Cell & Tissue Imaging Center
St. Jude Children's Research Hospital
332 North Lauderdale St., E7061
Memphis, TN 38105-2794
(901) 495-2536
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On 6/11/08 7:57 AM, "Sarah Aubut" <
[hidden email]> wrote:
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHello Listers,
I have seen a lot of posts discussing variouos nuclear stains, and I have a
question for you all.
I work with a Nikon Confocal system that has 3 lasers, a 405, 488 and 543. I
am having trouble finding a good nuclear stain that I can use on living
cells that will work with these lasers. I have been using hoescht, but it is
not optimal excited with the 405 laser. Are there any alternatives that
anyone knows of that can be used on living cells???
Thanks for your help!
Sarah Aubut