Re: Dynamic range of a CCD

Posted by Paul Maddox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Dynamic-range-of-a-CCD-tp593278p593282.html

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The noise is variation in the signal, which prohibits your ability to discriminate between signals varying less than this.  Dividing the FWC by less than this does not add to the ability to detect differences, even though technically the bit depth of the image could be higher.  Equally, dividing by greater than the noise results in “binned” values in the bit range which could, technically, be accurately detected as different values if the bit depth were greater.  Typically, if a camera has a noise of 7 electrons and a FWC of 21,000, then the dynamic range (your ability to discriminate to signals of different intensity) is 3000.  In this case, the manufacturer would set the bit-depth at 12 (4096).

 

The offset is subtracted from the full well capacity, this is set by the manufacturer (typically 50-200 counts in the image).  

 

There are many more detailed descriptions of this in various books (Video Microscopy by Inoue and Spring for instance).

 

Hope this helps

 

Paul S. Maddox, PhD
Assistant Professor
Institute for Research in Immunology and Cancer
Dept of Pathology and Cell Biol, U. de Montreal
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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gelman, Laurent
Sent: Wednesday, July 23, 2008 10:46 AM
To: [hidden email]
Subject: Dynamic range of a CCD

 

Hi everybody,

 

I don't understand why the dynamic range of a CCD is defined as the full-well capacity divided by the camera noise, and not minus the camera noise.

 

Thanks a lot for your help,

 

Laurent.

 

___________________________________________

Laurent Gelman, PhD

Friedrich Miescher Institut

Facility for Advanced Imaging and Microscopy

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CH-4058 Basel

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