Re: SP5 vs fluoview 1000 for FCS modification ?

Posted by Jean-Pierre CLAMME on
URL: http://confocal-microscopy-list.275.s1.nabble.com/SP5-vs-fluoview-1000-for-FCS-modification-tp593345p593347.html

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Hi,

First thank you for the infos.

Second do you need to go back through the scan heads ? Is it possible to
use another side port to extract the light for example to do cross
correlation ? I worked on two home made systems (FCS + imaging and
single molecule) were we would collect the light directly  after the
objective and redirect the signal to multiple APDs. On the SP5 we have
here we have external detectors. Could that kind of approach  be used ?

Thanks

JP

Michael Weber wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Jean-Pierre,
>
> on both systems one needs to install the external port modification
> (X1 / port 4 option), in order to bring the light out of the
> scan-head. Beside that, it's just another discussion about which
> system offers better transmission - dichromatic mirrors or AOBS.
>
> Gabor, can you control the APDs for imaging via the Leica software, or
> do you have to use an external solution?
>
> Michael
>
>
> Jean-Pierre CLAMME wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi,
>>
>> I'm wondering if onyone has experience with modifying a SP5 or a
>> Fluoview
>> 1000 for FCS mesures ? I work with Olympus stands before and they
>> generally
>> offer good access to external ports. But what a bout the SP5 ?  
>>
>> Thanks
>>
>> JP
>
>

--
Dr. Jean-Pierre CLAMME
Supervisor 2-Photon Imaging Facility
Dept. of Immunology, IMM-1, R310
The Scripps Research Institute
10550 North Torrey Pines Rd.
La Jolla, California 92037

Lab number: [phone]
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