Re: SP5 vs fluoview 1000 for FCS modification ?

Posted by Jean-Pierre CLAMME on
URL: http://confocal-microscopy-list.275.s1.nabble.com/SP5-vs-fluoview-1000-for-FCS-modification-tp593345p593349.html

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This is what we were doing on our system. We would choose  a point and
then move the table to bring that point under the parked beam.
I'm just wondering, is the fact  that scanning moves the beam  changing
the focal volume from one point to the other ? Or is it negligible ?

Thanks

JP
> The light path on the SP5 goes to the side port via the scan mirrors
> but not the spectral separation path. Going via the scan mirrors
> allows you to place the beam at a series of locations when collecting
> a set of FCS data (though you could do this by moving the stage with a
> simply parked beam I suppose).
This is what we were doing on our system. We would choose  a point and
then move the table to bring that point under the parked beam.
I'm just wondering Is the fact that when you use the scanner, you  but
the fact that when you use the scanner,


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