Re: SP5 vs fluoview 1000 for FCS modification ?

Posted by Sudipta Maiti on
URL: http://confocal-microscopy-list.275.s1.nabble.com/SP5-vs-fluoview-1000-for-FCS-modification-tp593345p593350.html

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

There is also a way to use external detectors, pretty much with any confocal
microscope, without worrying about alignment issues. We have used an
external APD to perform FCS at any chosen position in the image. More
details are in  "Fluorescence correlation microscopy with real-time
alignment readout", Kaushalya et al., Applied Optics, vol. 44 (2005) p.3262.
You may contact me off the list if you need to know more.
Sudipta

On Wed, 18 Jun 2008 16:24:49 -0700, Jean-Pierre CLAMME wrote

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> This is what we were doing on our system. We would choose  a point
> and then move the table to bring that point under the parked beam.
> I'm just wondering, is the fact  that scanning moves the beam  
> changing the focal volume from one point to the other ? Or is it
> negligible ?
>
> Thanks
>
> JP
> > The light path on the SP5 goes to the side port via the scan mirrors
> > but not the spectral separation path. Going via the scan mirrors
> > allows you to place the beam at a series of locations when collecting
> > a set of FCS data (though you could do this by moving the stage with a
> > simply parked beam I suppose).
> This is what we were doing on our system. We would choose  a point
> and then move the table to bring that point under the parked beam.
> I'm just wondering Is the fact that when you use the scanner, you  
> but the fact that when you use the scanner,
>
> >
> > [hidden email]
> > voice: [phone]
> > fax: [phone]
> >
> > http://som.flinders.edu.au/FUSA/Anatomy/
> > http://www.flinders.edu.au/neuroscience
> >

Dr. Sudipta Maiti
Associate Professor
Dept. of Chemical Sciences
Tata Institute of Fundamental Research
Homi Bhabha Raod, Colaba, Mumbai 400005
Ph. 91-22-2278-2716 / 2539
Fax: [phone]
alternate e-mail: [hidden email]
url: www.tifr.res.in/~biophotonics