bubbling media and imaging

Posted by Watkins, Simon C on
URL: http://confocal-microscopy-list.275.s1.nabble.com/bubbling-media-and-imaging-tp593362.html

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Hi folks, I in the middle of a bunch of experiments where I need to bubble gas into media while imaging (Going from normoxic to hypoxic media).  Obviously the bubbles perturb the quality of the DIC image.  I have tried all sorts of homemade diffusers to minimize the effect but to no avail... So has anyone of you developed a solution to this problem?  I could exchange the media, however regassing happens really quickly and the effects we are measuring are subtle.  Thus any changes in ionic concentrations or temperature may lead to a similar effect.

Ideas anyone

simon

 

Simon C. Watkins Ph.D, FRCPath

Professor and Vice Chair, Cell Biology and Physiology

Professor, Immunology

Director, Center for Biologic Imaging

BSTS 225, University of Pittsburgh

3500 Terrace St.

Pittsburgh PA 15261

Tel: [phone]

Fax:[phone]

URL: http://www.cbi.pitt.edu