Re: bubbling media and imaging

Posted by Beat Ludin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/bubbling-media-and-imaging-tp593362p593363.html

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Hi Simon

I would propose to use a closed perfusion chamber (yes, we sell one,
so there's some commercial interest here) and perfuse it with
pre-equilibrated medium. Since there is no fluid-gas interface in the
chamber, there will be no optical perturbation due to "ripples" on
the surface either. There are some other things to consider, so feel
free to contact me off the list.


Beat

At 15:13 28-07-2008, you wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>Hi folks, I in the middle of a bunch of experiments where I need to
>bubble gas into media while imaging (Going from normoxic to hypoxic
>media).  Obviously the bubbles perturb the quality of the DIC
>image.  I have tried all sorts of homemade diffusers to minimize the
>effect but to no avail... So has anyone of you developed a solution
>to this problem?  I could exchange the media, however regassing
>happens really quickly and the effects we are measuring are
>subtle.  Thus any changes in ionic concentrations or temperature may
>lead to a similar effect.
>Ideas anyone
>simon
>
>Simon C. Watkins Ph.D, FRCPath
>Professor and Vice Chair, Cell Biology and Physiology
>Professor, Immunology
>Director, Center for Biologic Imaging
>BSTS 225, University of Pittsburgh
>3500 Terrace St.
>Pittsburgh PA 15261
>Tel: [phone]
>Fax:[phone]
>URL: http://www.cbi.pitt.edu
>