Confocal Microscopy List

Re: bubbling media and imaging

Posted by Stephen Cody on
URL: http://confocal-microscopy-list.275.s1.nabble.com/bubbling-media-and-imaging-tp593362p593364.html

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Dear Simon,

Can you pre-bubble a reservoir and then pump (or gravity feed) this into your specimen bath?

Cheers Steve

Stephen H. Cody
Microscopy Manager
Central Resource for Advanced Microscopy
Ludwig Institute for Cancer Research
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Watkins, Simon C
Sent: Monday, 28 July 2008 11:13 PM
To: [hidden email]
Subject: bubbling media and imaging

Hi folks, I in the middle of a bunch of experiments where I need to bubble gas into media while imaging (Going from normoxic to hypoxic media). Obviously the bubbles perturb the quality of the DIC image. I have tried all sorts of homemade diffusers to minimize the effect but to no avail... So has anyone of you developed a solution to this problem? I could exchange the media, however regassing happens really quickly and the effects we are measuring are subtle. Thus any changes in ionic concentrations or temperature may lead to a similar effect.

Ideas anyone

simon

Simon C. Watkins Ph.D, FRCPath

Professor and Vice Chair, Cell Biology and Physiology

Professor, Immunology

Director, Center for Biologic Imaging

BSTS 225, University of Pittsburgh

3500 Terrace St.

Pittsburgh PA 15261

Tel: 412-352-2277

Fax:412-648-2797

URL: http://www.cbi.pitt.edu

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