Dear Simon,
there are several transparent plastics
which are permeable for gases. If the volume you image is really small and the
surface is really large, you might be able to avoid the bubbles by blowing oxygen
on the chamber to change the properties of the fluid inside. Ibidi.de (no
commercial interest) build chambers from such materials [these plastics can also
be used for DIC imaging], also ivss.de (again no commercial interest) use gas
permeable plastics.
Good luck, jens
From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Watkins, Simon
C
Sent: Monday, July 28, 2008 3:13 PM
To: [hidden email]
Subject: bubbling media and imaging
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the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi folks, I in the middle of a bunch of experiments where I
need to bubble gas into media while imaging (Going from normoxic to hypoxic
media). Obviously the bubbles perturb the quality of the DIC image.
I have tried all sorts of homemade diffusers to minimize the effect but to no
avail... So has anyone of you developed a solution to this problem? I
could exchange the media, however regassing happens really quickly and the
effects we are measuring are subtle. Thus any changes in ionic
concentrations or temperature may lead to a similar effect.
Ideas anyone
simon
Simon C. Watkins Ph.D, FRCPath
Professor and Vice Chair, Cell Biology and Physiology
Professor, Immunology
Director, Center for Biologic Imaging
BSTS 225, University of Pittsburgh
3500 Terrace St.
Pittsburgh PA 15261
Tel: 412-352-2277
Fax:412-648-2797
URL: http://www.cbi.pitt.edu
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