Re: dye and protein FRET
Posted by
David Stanek-3 on
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Hi Elisa,
It depends what you want to measure. For protein-protein we use mainly CFP-YFP and occasionally GFP-mRed and measure it by acceptor photobleaching and FLIM.
David
On Jun 24, 2008, at 5:52 PM, Elisa May wrote:
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Dear List,
which would be a recommended combination of fluorophore-fluorescent protein
for FRET?
Thanks a lot
Elisa
David Stanek, PhD.
Department of RNA biology
Institute of Molecular Genetics AS CR
Videnska 1083
142 20 Prague 4
Czech Republic
Tel.: +420-296443118
Fax: +420 224 310 955