Confocal Microscopy List

Re: Ask for help about LSM 510

Posted by Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Ask-for-help-about-LSM-510-tp593440p593449.html

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I'm not a Zeiss expert but I'd suggest that powering down the entire system

would have an equivalent effect to pressing any reset button. Then turn it

on following the normal sequence (which on most confocals means first

booting the computer, then turning on the microscope, scanhead and lasers

and finally starting the control software).

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
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Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of yee z
Sent: Friday, 18 July 2008 10:18 AM
To: [hidden email]
Subject: Re: Ask for help about LSM 510

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I did reboot the computer. It did not help.

It showed the same message again!

Almost cry!

And still can not find the reset button.

Is it possible that the software requires turning on the computer before LSM system?

One of old confocal system I used required such things.

If you ignite laser first, the software would not be opened.

The technician did not come today.

Hopefully he will come tomorrow.

--- On Thu, 7/17/08, Vickery Trinkaus-Randall <[hidden email]> wrote:

From: Vickery Trinkaus-Randall <[hidden email]>
Subject: Re: Ask for help about LSM 510
To: [hidden email]
Date: Thursday, July 17, 2008, 6:16 PM

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

simple
I think that you may have been moving too quickly with pushing all of 
your button!
make sure that it is connecting before you ask it to scan
Just exit and reboot and you will be fine!

Yi Zheng wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi everyone!
>
>Here is an emergency.
>
>I am doing my data analysis on zeiss LSM 510 while the whole system is 
>runing. I mean all laser are on and I was collecting data.
>
>The software was down suddenly.
>So I quit it and tried to restart it. 
>Then I got the message like "Fail to connect to realtime controller.
If the 
>system is running open the door "reset RTC" on the front of the
electronics 
>rack. press the "Reset" button and wait 30 seconds.
>But the problem is I can not find any door or reset button behind it.
>
>I called Zeiss, but their system is down too!
>
>Is there anyone here can help?
>
>
>  
>

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